Onset of neurophysin self‐association upon neurophysin/neuropeptide hormone precursor biosynthesis

FEBS Letters

Tamaoki, Hidetsune³  Brownstein, Michael J.²  Chaiken, Irwin M.³  Gainer, Harold¹ 

[1] Laboratory of Neurochemistry and Neuroimmunology, National Institute of Child Health and Human Development, Bethesda, MD 20205, USA;Laboratory of Cell Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20205, USA;Laboratory of Chemical Biology, National Institute of Arthritis, Building 10, Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20205, USA

关键词: Neuropeptide hormone;    Neurophysin;    Prohormone association;    Hormone—protein interaction;    Quantitative affinity chromatography;    BNP-II;    bovine neurophysin II (vasopressin-associated);    NP;    neurophysin;    LVP and AVP;    lysine and arginine vasopressin;    OT;    oxytocin;    pro-NP/AVP;    pulse-labelled biosynthetic precursor for AVP and AVP-associated NP;    pro-NP/OT;    pulse-labelled biosynthetic precursor for OT and OT-associated NP;    K NPL;    dissociation constant of unliganded;    soluble NP for unliganded;    immobilized NP;    K NPLLdissociation constant of liganded;    soluble NP for liganded;    immobilized NP;    K proNPL;    dissociation constant of rat pro-NP/harmone for unliganded;    immobilized NP;   

DOI  :  10.1016/0014-5793(83)80317-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The potential of the common biosynthetic precursor of neurophysin and neuropeptide hormones to self-associate has been assessed by quantitative affinity chromatographic analysis. The precursor form, with the hormone sequence in the amino terminal region and assumed able to interact intramolecularly with the hormone binding site of the neurophysin domain of the folded precursor, exhibits an affinity for neurophysin-agarose which is intermediate between those of unliganded neurophysin and non-covalently hormone-liganded neurophysin. The results lead to a prediction that neurophysin self-association is established upon precursor synthesis and prior to limited proteolysis of the precursor to release mature neurophysin and hormone components. Such self-association could play a role in packaging of the precursor into secretory granules and in regulating subsequent precursor processing events within the granules.

【 授权许可】

Unknown