Characterisation of an intermediate in neurophysin biosynthesis in the guinea pig

FEBS Letters

Robinson, Iain C.A.F.¹  Gordon-Weeks, Ruth¹  Jones, Peter M.¹ 

[1] Laboratory of Endocrine Physiology and Pharmacology, National Institute for Medical Research, Mill Hill, London NW 7 1AA, England

关键词: AVP;    Precursor;    Neurophysin;    Glycopeptide;    Biosynthesis;    Intermediate;   

DOI  :  10.1016/0014-5793(83)80845-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In addition to oxytocin (OT), vasopressin (AVP) and their respective neurophysins (NPs), another [³⁵S]cysteine incorporating component is present in the guinea pig neurohypophysis. Gel filtration and Con A affinity chromatography revealed that this component was larger than NP and was glycosylated. NP-immunoreactivity was assessed using antisera which distinguish the OT- and AVP-related NPs. Whilst the anti-NP antiserum detected only one component (guinea pig NP), the anti-NP antiserum detected both NP and the glycosylated ³⁵S-labelled component. These results suggest that a significant amount of NP in guinea pig neural lobes bears a glycopeptide extension and represents a partially processed form of the AVP precursor in this species.

【 授权许可】

Unknown