Analysis of the 5′ non‐coding region of rat liver S‐adenosylmethionine synthetase mRNA and comparison of the M r deduced from the cDNA sequence and the purified enzyme

FEBS Letters

Alvarez, Luis¹  Asunción, Miryam¹  Corrales, Fernando¹  Pajares, Maria A.¹  Mato, José M.¹ 

[1] Instituto de Investigaciones Biomédicas del CSIS, Arturo Duperier 4, Madrid 28029, Spain

关键词: S-Adenosylmethionine synthetase;    cDNA sequence;    Sulfhydryl groups;    Messenger RNA;    Rat liver;    Ruman liver;   

DOI  :  10.1016/0014-5793(91)81245-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 3 kb cDNA coding for rat liver S-adenosylmethionine (AdoMet) synthetase has been isolated. The M _r of the protein has been unequivocally determined by cDNA sequencing and enzyme purification on a thiopropyl-Sepharose column. The length of the mRNA 5′ non-coding region has been defined by primer-extension analysis. The rat liver cloned cDNA has been also used to detect S-adenosylmethionine synthetase mRNA in human liver.

【 授权许可】

Unknown