Expression of non‐hepatic‐type S‐adenosylmethionine synthetase isozyme in rat hepatomas induced by 3′‐methyl‐4‐dimethylaminoazobenzene

FEBS Letters

Hirokawa, Saburo¹  Terashima, Hiromichi³  Kobayashi, Yuki¹  Tsukada, Kinji¹  Sugiyama, Toshihiro²  Wada, Kenji¹ 

[1] Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Kanda-surugadai, Chiyoda-ku, Tokyo 101, Japan;First Department of Biochemistry, Akita University School of Medicine, Hondo, Akita 010, Japan;Department of Molecular Genetics, Nippon Roche Research Center, 200 Kajiwara, Kamakura, Japan

关键词: S-Adenosylmethionine synthetase;    Isozyme;    Carcinogenesis;    Hepatocellular carcinoma;   

DOI  :  10.1016/0014-5793(93)81682-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is known that a high incidence of hepatocellular carcinoma in rat liver can be induced by such azo dye carcinogens as 3′-methyl-4-dimethylaminoazobenzene (3′-MeDAB). Mammalian S-adenosylmethionine (AdoMet) synthetase exists as two isozymes, non-hepatic-type and liver-type enzymes, which are the products of two different genes. We have examined the expression of two AdoMet synthetase isozyme proteins and mRNAs in rat hepatomas induced by 3′-Me-DAB. The levels of non-hepatic-type enzyme protein and mRNA are clearly induced by 3′-Me-DAB feeding. On the other hand, the levels of liver-type enzyme protein and mRNA are nearly the same or slightly decreased during hepatocarcinogenesis. These results indicate that the expression of the non-hepatic-type isozyme gene is obviously influenced with the progression of carcinogenesis and that the non-hepatic-type isozyme is useful as a oncodevelopmental marker in the liver.

【 授权许可】

Unknown