FEBS Letters | |
Expression of non‐hepatic‐type S‐adenosylmethionine synthetase isozyme in rat hepatomas induced by 3′‐methyl‐4‐dimethylaminoazobenzene | |
Hirokawa, Saburo1  Terashima, Hiromichi3  Kobayashi, Yuki1  Tsukada, Kinji1  Sugiyama, Toshihiro2  Wada, Kenji1  | |
[1] Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Kanda-surugadai, Chiyoda-ku, Tokyo 101, Japan;First Department of Biochemistry, Akita University School of Medicine, Hondo, Akita 010, Japan;Department of Molecular Genetics, Nippon Roche Research Center, 200 Kajiwara, Kamakura, Japan | |
关键词: S-Adenosylmethionine synthetase; Isozyme; Carcinogenesis; Hepatocellular carcinoma; | |
DOI : 10.1016/0014-5793(93)81682-P | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
It is known that a high incidence of hepatocellular carcinoma in rat liver can be induced by such azo dye carcinogens as 3′-methyl-4-dimethylaminoazobenzene (3′-MeDAB). Mammalian S-adenosylmethionine (AdoMet) synthetase exists as two isozymes, non-hepatic-type and liver-type enzymes, which are the products of two different genes. We have examined the expression of two AdoMet synthetase isozyme proteins and mRNAs in rat hepatomas induced by 3′-Me-DAB. The levels of non-hepatic-type enzyme protein and mRNA are clearly induced by 3′-Me-DAB feeding. On the other hand, the levels of liver-type enzyme protein and mRNA are nearly the same or slightly decreased during hepatocarcinogenesis. These results indicate that the expression of the non-hepatic-type isozyme gene is obviously influenced with the progression of carcinogenesis and that the non-hepatic-type isozyme is useful as a oncodevelopmental marker in the liver.
【 授权许可】
Unknown
【 预 览 】
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