Uteroglobin, an apically secreted protein of the uterine epithelium, is secreted non‐polarized from MDCK cells and mainly basolaterally from Caco‐2 cell

FEBS Letters

Vogel, Lotte Katrine¹  Norén, Ove¹  Suske, Guntram²  Sjöström, Hans¹  Beato, Miguel² 

[1] Department of Medical Biochemistry and Genetics, Biochemistry Laboratory C, University of Copenhagen, The Panum Institute, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark;Institut für Molekularbiologie und Tumorforschung, E.-Mannkopff-Str. 2, D-3550 Marburg, Germany

关键词: Uteroglobin;    MDCK: Caco-2;    Sorting;    Non-polarized;    Aminopeptidase N;   

DOI  :  10.1016/0014-5793(93)80891-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A complete cDNA encoding rabbit Uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete Uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete Uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secrete Uteroglobin in a non-sorted manner. It has, however, previously been shown that Uteroglobin is secreted exclusively at the apical membrane in primary cell culture of endometrial epithelial cells [S.K. Mani et al. (1991) Endocrinology 128, 1563-1573]. This suggests that either the endometrial epithelium has an apical default pathway or recognises a sorting signal not recognised by MDCK cells and Caco-2 cells. Our data thus show that a soluble molecule can be secreted at the apical, the basolateral or both membranes depending on the cell type.

【 授权许可】

Unknown