Prooxidant role of vitamin E in copper induced lipid peroxidation

FEBS Letters

Roveri, Antonella¹  Zamburlini, Adriana¹  Maiorino, Matilde¹  Ursini, Fulvio² 

[1] Department of Biological Chemistry, via Trieste 75, 35121 Padova, Italy;Department of Chemical Science and Technology, via Cotonificio 108, 33100 Udine, Italy

关键词: Vitamin E;    α-Tocopherol;    Copper;    Phospholipid hydroperoxide;    Low density lipoprotein;    Atherosclerosis;    DOC;    sodium deoxycholate;    HPLC;    high-performance liquid chromatography;    LDL;    low density lipoproteins;    PLPC;    palmitoyl linoleoyl phosphatydilcoline;    PBS;    10 mM Na-Phosphate buffer;    0.16 M NaCl;    pH 7.4;    PLPCOOH;    palmitoyl linoleoyl phosphatydilcoline 13-hydroperoxide;   

DOI  :  10.1016/0014-5793(93)80267-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When exposed to Cu²⁺, α-tocopherol, in detergent dispersion, is rapidly oxidised. Moreover, if phospholipids and traces of their hydroperoxide derivatives are included in these dispersions, Cu²⁺ initiates lipid peroxidation, the rate of which is dramatically stimulated by α-tocopherol. The observation that the rate of α-tocopherol consumption is identical in the absence and in the presence of lipids undergoing peroxidation, apparently rules out any antioxidant effect. These results are consistent with a prooxidant effect of vitamin E, mediated by its capability to reduce Cu²⁺ to Cu⁺ which, in turn, produces, from lipid hydroperoxides, the highly reactive alkoxyl radicals. Present data highlight the risk of misleading results in interpreting the significance of lags in peroxidation of LDL challenged with Cu²⁺.

【 授权许可】

Unknown