Reactions of the α‐tocopheroxyl radical in micellar solutions studied by nanosecond laser flash photolysis

FEBS Letters

Bisby, Roger H.²  Parker, Anthony W.¹ 

[1] Laser Support Facility, Rutherford Appleton Laboratory, Science and Engineering Research Council, Chilton, Didcot, Oxon, OX11 0QX, UK;Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK

关键词: α-Tocopherol;    Vitamin E;    Radical;    Ascorbate;    Flash photolysis;    Micelle;    HTAC;    hexadecyltrimethyl ammonium chloride;    SDS;    sodium dodecyl sulphate;    α-Toc-OH;    α-tocopherol;   

DOI  :  10.1016/0014-5793(91)81260-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Laser flash photolysis of α-tocopherol in methanol and in aqueous micellar solutions has been shown to produce the α-tocopheroxyl radical. The reaction between the α-tocopheroxyl radical and ascorbate in positively charged hexadecyltrimethylammonium chloride (HTAC) micelles occurred with a second order rate constant of 7.2 × 10⁷ M⁻¹·s⁻¹, whereas in negatively charged sodium dodecyl sulphate (SDS) micelles the rats constant was only 3.8 × 10⁴ M⁻¹·s⁻¹. The α-tocopheroxyl radical was found to be relatively long-lived in HTAC micelles (t_½ ⩾ 5 min), allowing the slow disappearance of the α-tocopheroxyl radical by reaction with glutathione to be observed.

【 授权许可】

Unknown