Inhibition of 5‐lipoxygenase by vitamin E

FEBS Letters

Reddanna, Pallu¹  Krishna Rao, M.¹  Channa Reddy, C.¹ 

[1] Center for Air Environment Studies and Department of Veterinary Science, The Pennsylvania State University, University Park, PA 16802, USA

关键词: 5-Lipoxygenase;    Vitamin E;    α-Tocopherol;    Enzyme inhibition;    Antioxidant;    Leukotriene;   

DOI  :  10.1016/0014-5793(85)80075-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified 5-lipoxygenase from potato tubers was inhibited strongly by vitamin E and its analogs. The inhibition by d-α-tocopherol was found to be irreversible and non-competitive with respect to arachidonic acid. An IC₅₀ of 5μM was calculated for d-α-tocopherol. The inhibition appears to be unrelated to its antioxidant function. Binding studies with ¹⁴C-labelled d-α-tocopherol revealed that there is a strong interaction between vitamin E and 5-lipoxygenase. Tryptic digestion and peptide mapping of 5-lipoxygenase-vitamin E complex indicate that vitamin E binds strongly to a single peptide. These studies suggest that cellular vitamin E levels may have profound influence on the formation of leukotrienes.

【 授权许可】

Unknown