FEBS Letters | |
Inhibition of 5‐lipoxygenase by vitamin E | |
Reddanna, Pallu1  Krishna Rao, M.1  Channa Reddy, C.1  | |
[1] Center for Air Environment Studies and Department of Veterinary Science, The Pennsylvania State University, University Park, PA 16802, USA | |
关键词: 5-Lipoxygenase; Vitamin E; α-Tocopherol; Enzyme inhibition; Antioxidant; Leukotriene; | |
DOI : 10.1016/0014-5793(85)80075-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Purified 5-lipoxygenase from potato tubers was inhibited strongly by vitamin E and its analogs. The inhibition by d-α-tocopherol was found to be irreversible and non-competitive with respect to arachidonic acid. An IC50 of 5μM was calculated for d-α-tocopherol. The inhibition appears to be unrelated to its antioxidant function. Binding studies with 14C-labelled d-α-tocopherol revealed that there is a strong interaction between vitamin E and 5-lipoxygenase. Tryptic digestion and peptide mapping of 5-lipoxygenase-vitamin E complex indicate that vitamin E binds strongly to a single peptide. These studies suggest that cellular vitamin E levels may have profound influence on the formation of leukotrienes.
【 授权许可】
Unknown
【 预 览 】
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