A novel human tyrosine kinase gene inducible in T cells by interleukin 2

FEBS Letters

Ohtani, Kiyoshi¹  Tanaka, Nobuyuki¹  Nakamura, Masataka¹  Sugamura, Kazuo¹  Asao, Hironobu¹ 

[1] Department of Microbiology, Tohoku University School of Medicine, Sendai 980, Japan

关键词: Protein tyrosine kinase;    Molecular cloning;    Interleukin 2;    T cell;    IL-2;    interleukin 2;    IL-2R;    interleukin 2 receptor;    SH2;    Src homology 2;    SH3;    Src homology 3;    PBL;    peripheral blood lymphocytes;    PHA;    phytohemagglutinin;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    HTLV-I;    human T-cell leukemia virustype-I;   

DOI  :  10.1016/0014-5793(93)81520-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned a novel human protein tyrosine kinase gene specific to T cells by the polymerase chain reaction method. This gene encodes a 620 amino-acid polypeptide including a catalytic domain for tyrosine kinase, an SH2 domain and an SH3 domain, seemingly belonging to the src family. However, characteristics of a long unique N-terminal stretch and lack of a myristylation site at the N-terminus and of a kinase regulatory tyrosine residue in the C-terminus classify this molecule into a new subfamily comprising recently cloned mouse tec, itk/tsk and human atk/bpk genes. This gene was transcriptionally induced in normal T cells by interleukin 2 stimulation. These results suggest the crucial roles of this gene in T cell proliferation and differentiation.

【 授权许可】

Unknown