Interleukin 2 mediates p72 sykactivation in peripheral blood lymphocytes

FEBS Letters

Yang, Cheng¹  Sada, Kiyonao¹  Qin, Suofu¹  Yamamura, Hirohei¹  Inazu, Tetsuya¹  Taniguchi, Takanobu¹ 

[1] Department of Biochemistry, Fukui Medical School, Matsuoka, Fukui 910-11, Japan

关键词: Interleukin 2;    Protein-tyrosine kinase p72 syk;    Peripheral blood lymphocyte;    PTK;    protein-tyrosine kinase;    HBSS;    Hanks' balanced salt solution;    IL-2;    interleukin 2;    IL-2R;    interleukin 2 receptor;    PBLs;    peripheral blood lymphocytes;    [Ca2+]i;    intracellular calcium concentration;    BAPTA-AM;    acetoxymethyl esters of 5;    5′-diethyl-bis-(O-aminophenoxy)-N′;    N;    N′;    N′-tetraacetic acid;   

DOI  :  10.1016/0014-5793(94)00450-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of interleukin 2 (IL-2) to stimulate p72 ^syk activity in intact porcine peripheral blood lymphocytes was examined. We demonstrated that IL-2 activated p72 ^syk in a time- and dose-dependent number, for which its peak time and maximum responsive dose were 5 min and 100 U/ml, respectively. This activation was observed only in cytosolic fractions and not in membrane ones. However, IL-2 failed to induced calcium mobilization. Moreover, IL-2-inducible p72 ^syk activation was not affected when extra and intracellular calcium was depleted. These data suggest that the IL-2 signaling pathways through p72 ^syk in peripheral blood lymphocytes is different, at least in part, from other agonist, such as concanavalin A in polymorphonuclear neutrophils which can trigger both the activation of p72 ^syk and intracellular calcium mobilization.

【 授权许可】

Unknown