Bromelain protease F9 reduces the CD44 mediated adhesion of human peripheral blood lymphocytes to human umbilical vein endothelial cells

FEBS Letters

Maurer, H.R.¹  Eckert, K.¹  Harrach, T.¹  Graf, H.²  Munzig, E.¹ 

[1] Institut für Pharmazie der Freien Universität Berlin, Kelchstr 31, 12169 Berlin, Germany;Schering AG, Pharma-Forschung, D-13342 Berlin, Germany

关键词: Bromelain F9;    CD44;    Adhesion;    Peripheral blood lymphocyte;    Human umbilical vein edothelial cell;   

DOI  :  10.1016/0014-5793(94)00860-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thiol protease bromelain has been shown to remove T-cell CD44 molecules from lymphocytes and to affect T-cell activation. We investigated the effect of a highly purified bromelain protease F9 (F9) on the adhesion of peripheral blood lymphocytes (PBL) to human umbilical vein endothelial cells (HUVEC). Preincubation of the lymphocytes with F9 reduced the adherence to about 20% of unstimulated and to about 30% of phorboldibutyrate (P(Bu)₂) stimulated lymphocytes. Using flow cytometry, both crude bromelain and protease F9 reduced the expression of CD44, but not of LFA-1, on PBL. F9 was about 10 times more active than crude bromelain; at 2.5 μg/ml of F9 about 97% inhibition of CD44 expression was found. A mAb against CD44 was tested and found to block the F9-induced decrease in PBL-binding to HUVEC. The results indicate that F9 selectively decreases the CD44 mediated binding of PBL to HUVEC.

【 授权许可】

Unknown