Detection of minor adducts in cisplatin‐modified DNA by transcription footprinting

FEBS Letters

Leng, Marc¹  Locker, Daniel¹  Decoville, Martine¹  Schwartz, Annie¹ 

[1] Centre de Biophysique Moléculaire, CNRS, 1A avenue de la recherche scientifique 45071, Orléans Cedex 2, France

关键词: cis-Diamminedichloroplatinum(II);    Adduct;    T7 RNA polymerase;    Sodium cyanide;   

DOI  :  10.1016/0014-5793(93)81447-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two DNA restriction fragments containing either a d(GC)₅ or a d(TTGCTTGATTAGTTGTGTT) insert were subjected to reaction with cis-diamminedichloroplatinum(II) and were then used as templates for RNA synthesis by T7 RNA polymerase. Within the d(GC)₅ insert, interstrand cross-links are preferentially formed. Within the second insert, the reactivity order of the potential binding sites is d(ApG) > d(GpC/ GpC) = d(GpA) > d(GpTpG). In the presence of cyanide ions, the adducts are much less stable at the d(GpA) sites than at the d(GpCpG) sites, in double-stranded DNA.

【 授权许可】

Unknown