Effects of 1,3‐chelation induced by cis‐diamminedichloroplatinum(II) on the stability of DNA duplexes

FEBS Letters

Akagi, Masao¹  Tamura, Masahiro¹  Urata, Masayuki¹  Urata, Hidehito¹ 

[1] Osaka University of Pharmaceutical Sciences, 2-10-65 Kawai, Matsubara, Osaka 580, Japan

关键词: cis-Diamminedichloroplatinum(II);    Decanucleotide duplex;    1;    3-Intra-strand cross-link;    Melting temperature T m;    cis-DDP;    cis-diamminechloroplatinum(II);    T m;    melting temperature;   

DOI  :  10.1016/0014-5793(92)81116-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several 1,3-intra-strand cross-linked decadeoxynucleotide duplexes, modified with cis-diamminedichloroplatinum(II) (cis-DDP), and their base substitution analogues at the complementary site to the intervening base of the coordination sites, were synthesized and measured for UV-melting, profiles to determine melting temperature (T _m) values. The results indicated the thermal stability of the oligonucleotide duplexes containing Pt-induced 1,3-intra-strand cross-linking did not depend on the kind of intervening base of the coordination site but rather on its complementary base. These results may explain the mutagenicity or cis-DDP from a chemical aspect.

【 授权许可】

Unknown