Identification of a N7-guanine adduct of 1-bromopropane in calf thymus DNA by mass spectrometry

Molecular & Cellular Toxicology

Eung-Seok Lee¹  Eun-Kyung Kim¹  Keumhan Noh¹  Mi Jeong Kang¹  Pritam Thapa¹  Tae Cheon Jeong¹  Hye Gwang Jeong²  Ki Sun Jeong¹  Sangkyu Lee³  Jun-Ho Lee⁴  Mahesh Raj Nepal¹ 

[1] Yeungnam University$$;Chungnam National University$$;Kyungpook National University$$;Daejeon University$$

关键词: Adduct formation;   

DOI  :  10.1007/s13273-016-0002-5

学科分类：分子生物学，细胞生物学和基因

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

As a replacement for chlorofluorocarbons that cause ozone depletion, 1-bromopropane has been widely used in work place. In the present study, the formation of N⁷-guanine adduct in DNA by 1-bromopropane was evaluated in vitro to elucidate the possible mechanism of its toxic action. N⁷-Propyl guanine was chemically synthesized and structurally characterized by NMR, UV, HPLC, and liquid chromatographyelectrospray ionization mass spectrometry (LC- ESI MS) for using as a reference standard. An incubation of 2ʹ-deoxyguanosine with 1-bromopropane produced N⁷-propyl adduct, which was identified by UV, HPLC and ESI-MS. In addition, N7-guanine adduct was also identified from the incorporation of calf thymus DNA with 1-bromopropane at the physiological condition by LC-ESI MS. Furthermore, the production of adduct was proportional to the amounts of 1-bromopropane used. These results indicated that the molecular mechanism underlying toxic effects of 1-bromopropane would be associated with the adduct formation on DNA at least in part.

【 授权许可】

Unknown