FEBS Letters | |
Inhibitory mechanism of sinapinic acid against peroxynitrite‐mediated tyrosine nitration of protein in vitro | |
Osawa, Toshihiko3  Kato, Yoji2  Niwa, Toshio1  Doi, Umeyuki1  | |
[1] Department of Research and Development, San-ei Sucrochemical Co., Ltd., Chita 478-8503, Japan;School of Humanities for Environmental Policy and Technology, Himeji Institute of Technology, Himeji 670-0092, Japan;Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan | |
关键词: Peroxynitrite; Sinapinic acid; Adduct; Nitrotyrosine; Phenolic antioxidant; NMR; nuclear magnetic resonance; LC-MS; liquid chromatography-mass spectrometry; ML; mono-lactone type dimer; DL; di-lactone type dimer; | |
DOI : 10.1016/S0014-5793(99)01216-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The peroxynitrite-scavenging ability of some phenolic antioxidants, p-coumaric acid, caffeic acid and sinapinic acid, was examined and compared with ascorbic acid and tocopherol using 3-nitrotyrosine formation as a marker. Among these, caffeic acid and sinapinic acid strongly inhibited the formation of 3-nitrotyrosine in protein. The treatment of protein with peroxynitrite in the presence of sinapinic acid, but not caffeic acid, produced a novel product determined by reversed-phase high performance liquid chromatography (HPLC). The product formed was purified and then identified as a mono-lactone type dimer (ML) of sinapinic acid by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS). This ML was converted from a di-lactone type dimer, obtained from sinapinic acid with peroxidase/hydrogen peroxide, in neutral buffer. In this report, we have proposed that the ML of sinapinic acid is generated via one-electron oxidation by peroxynitrite treatment.
【 授权许可】
Unknown
【 预 览 】
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