Interaction of carbonyl cyanide m‐chlorophenylhydrazone with the photosystem II acceptor side

FEBS Letters

Samuilov, Vitaly D.¹  Barsky, Eugene L.¹ 

[1] Department of Cell Physiology and Immunology, Moscow State University, Moscow 119899, Russian Federation

关键词: Chloroplast;    Electron transport;    Photosystem II;    ADRY agent;    CCCP;    ADRY;    acceleration of the deactivation reactions of the water-splitting enzyme system Y;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-p-benzoquinone;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    DNP-INT;    2-iodo-6-isopropyl-3-methyl-2';    4;    4'-trinitrodiphenyl ether;    FeCy;    potassium ferricyanide;    HQNO;    2-n-heptyl-4-hydroxyquinoline N-oxide;    (Mn)4;    the tetranuclear Mn cluster of water-splitting complex Y;    P680;    the reaction center of photosystem II;    Pheo;    the intermediate electron acceptor of photosystem II (pheophytin a);    Qa and qb;    primary and secondary plastoquinones of photosystem II;    Qc and Qz;    plastoquinone binding sites in the cytochrome blf complex;    Qp;    membrane pool of plastoquinone;    SiMo;    sodium silicomolybdate;    TMPD;    N;    N;    N';    N'-tetiamettiyl-p-pheaylenediamme;    Yz;    tyrosine-161 of the photosystem II Dl polypeptide;   

DOI  :  10.1016/0014-5793(93)80074-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that CCCP, known as an uncoupler of photophosphorylation and an ADRY agent, inhibits FeCy photoreduction and coupled O₂ evolution by isolated chloroplasts equally (1₅₀ ∼ 2μM), but is practically without effect on the O₂ evolution coupled with SiMo reduction within the 0.2–10μM concentration range. CCCP has no effect on the nanosecond chlorophyll fluorescence in chloroplasts incubated at low light intensity, but decreases it at high light intensity. The electron transfer from reduced TMPD or duroquinol to methylviologen is resistant to CCCP. The efficiency of the CCCP inhibitory action on the FeCy photoreduction depends on the rate of electron flow, which is controlled by the light intensity. The data obtained show that CCCP is oxidized by the photosystem II donor side and is reduced by Q_p, competing for electrons with FeCy and the cytochrome blf complex.

【 授权许可】

Unknown