Photoreduction of silicomolybdate in chloroplasts by agents accelerating the deactivation reactions of the water‐oxidizing system

FEBS Letters

Samuilov, Vitaly D.¹  Klimov, Vyacheslav V.²  Gubanova, Olga N.²  Barsky, Eugene L.¹  Kozlov, Yury N.² 

[1] Department of Cell Physiology and Immunology, Moscow State University, Moscow 119899, Russian Federation;Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, Pushchino 142292, Russian Federation

关键词: Photosystem II;    Silicomolybdate;    ADRY agent;    Uncoupler;    Cyclic voltammetry;    ADRY;    acceleration of the deactivation reactions of the water-splitting enzyme system Y;    ANT2p;    2-(3-chloro-4-trifluoromethyl)anilino-3;    5-dinitrothiophene;    CCCP;    carbonyl cyanide chlorophenylhydrazone;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    FeCy;    potassium ferricyanide;    P680;    the reaction center of photosystem II;    PCP;    2;    3;    4;    5;    6-pentachlorophenol;    PSII;    photosystem II;    SiMo;    silicomolybdate;    TTFB;    4;    5;    6;    7-tetrachloro-2-trifluoromethylbenzimidazole;   

DOI  :  10.1016/0014-5793(94)01309-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uncouplers of photosynthetic phosphorylation, CCCP, TTFB and PCP, inhibited light-induced O₂ evolution in the Hill reaction with SiMo (I ₅₀ ∼20, 3 and 45 μM, respectively), but only insignificantly diminished SiMo photoreduction by pea chloroplasts. The same properties were exhibited by the ADRY agent ANT2p. CCCP, TTFB and PCP are oxidizable compounds with redox potentials of +1.17, +1.18 and +1.09 V (pH 6.0), as determined by cyclic voltammetry. Similarly to NH₂OH, the tested uncouplers can apparently serve as electron donors for photosystem II.

【 授权许可】

Unknown