Functional size of the thylakoid phosphatases determined by radiation inactivation

FEBS Letters

Pan, Rong Long¹  Tzeng, Chi Meng¹  Hui Hsu, Lu¹ 

[1] Institute of Radiation Biology and Department of Nuclear Science, College of Nuclear Science, National Tsing Hua University, Hsin Chu 30043, Taiwan, Republic of China

关键词: Phosphatase;    Thylakoid;    Functional size;    Radiation inactivation;    Chl;    chlorophyll;    LHC II;    light-harvesting chlorophyll protein complex of PS II;   

DOI  :  10.1016/0014-5793(93)81314-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Radiation inactivation technique was employed to determine the functional size of phosphatases from thylakoid membrane. The enzymatic activities of phosphatases decayed in a simple function with the increase of radiation dosage. D₃₇ values of 18.8 ± 2.4-14.1 ± 1.5 Mrad were obtained, using phosphoserine, phosphothreonine, p-nitrophenol phosphate, and phospho-histone V-S, respectively, as substrates. The molecular masses of 48.2 ± 6.3-61 ± 5.7 kDa were yielded by target theory analysis. We thus speculate that the thylakoid alkaline phosphatase is probably a monomer while acid phosphatase is functionally a dimer in situ.

【 授权许可】

Unknown