Functional size analysis of pyrophosphatase from Rhodospirillum rubrum determined by radiation inactivation

FEBS Letters

Wu, Jia Jiu¹  Ma, Jing Tyan¹  Pan, Rong Long¹ 

[1] Institute of Radiation Biology and Nuclear Science, College of Nuclear Science, National Tsing Hua University, Hsinchu 30043, Taiwan, Republic of China

关键词: Functional size;    Radiation inactivation;    Pyrophosphatase;    Chromatophore;    Rhodospirillum rubrum;    BChl;    bacteriochlorophyll;    PPase;    pyrophosphatase;    PPi;    inorganic pyrophosphate;   

DOI  :  10.1016/0014-5793(91)80552-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A radiation inactivation technique was employed to determine the functional size of pyrophosphatase (PPase) from the chromatophores of Rhodospirillum rubrum. The activities of hydrolysis and synthesis reactions of pyrophosphatase and its coupled proton translocation decayed in a simple exponential function with the increase of radiation dosages. D ₃, values of 5.2 ± 0.7 and 5.8 ± 0.8 Mrads were obtained for pyrophosphate hydrolysis and its associated proton translocation yielding molecular masses of 167.7 ± 30.7 and 156.3 ± 26.6 kDa, respectively. Similarly, a D ₃, value of 4.4 ± 0.6 Mrads was measured for the acid-base induced pyrophosphate synthesis resulting in a radiation sensitive size of 196.3 ± 31.9 kDa.

【 授权许可】

Unknown