Radiation inactivation analysis of H+‐pyrophosphatase from submitochondrial particles of etiolated mung bean seedlings

FEBS Letters

Yang, Su Jing²  Pan, Rong Long¹  Jiang, Shih Sheng¹  Kuo, Soong Yu¹ 

[1] Department of Life Science, College of Life Science, National Tsing Hua University, Hsin Chu 30043, Taiwan;Department of Radiological Technology, Chungtai Institute of Health Science and Technology, Taichung 40605, Taiwan

关键词: Functional size;    Radiation inactivation;    H+-pyrophosphatase;    Proton translocation;    Submitochondrial particle;    BSA;    bovine serum albumin;    EDTA;    ethylenediaminetetraacetic acid;    EGTA;    ethyleneglycol-bis-(aminoethyl ether) N;    N;    N′;    N′-tetraacetic acid;    PPase;    pyrophosphatase;   

DOI  :  10.1016/S0014-5793(00)01215-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Radiation inactivation analysis was employed to determine the functional masses of enzymatic activity and proton translocation of H⁺-pyrophosphatase from submitochondrial particles of etiolated mung bean seedlings. The activities of H⁺-pyrophosphatase decayed as a simple exponential function with respect to radiation dosage. D ₃₇ values of 6.9±0.3 and 7.5±0.5 Mrad were obtained for pyrophosphate hydrolysis and its associated proton translocation, yielding molecular masses of 170±7 and 156±11 kDa, respectively. In the presence of valinomycin and 50 mM KCl, the functional size of H⁺-pyrophosphatase of tonoplast was decreased, while that of submitochondrial particles remained the same, indicating that they are two distinct types of proton pump using PP_i as their energy source.

【 授权许可】

Unknown