Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells

FEBS Letters

Pignataro, Omar²  de Asua, Luis Jimenez¹  Goin, Mercedes¹ 

[1] Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Obligado 2490, 1428, Buenos Aires, Argentina;Instituto de Biologia y Medicina Experimental (IBYME), Obligado 2490, 1428, Buenos Aires, Argentina

关键词: Prostaglandin F2α;    R 59022;    Diacylglycerol kinase;    Protein kinase C;    DNA synthesis;    PGF2α;    prostaglandin F2α;    PKC;    protein kinase C;    DAG;    diacylglycerol;    DGK;    diacylglycerol kinase;    R 59022;    6-[2-(4-[(4-fluoro phenyl) phenylmethylene]-1-piperdinyl]-1-methyl)5H-thiazolo[3;    2-α]-pyrimidin-5-one.;   

DOI  :  10.1016/0014-5793(93)81738-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

R59022, a diacylglycerol kinase inhibitor, enhances the prostaglandin F2α (PGF2α)-induced diacylglycerol (DAG) synthesis in Swiss 3T3 cells. It also potentiates the PGF2α-mediated protein kinase C (PKC)-dependent 80 kDa protein (80K) phosphorylation and initiation of DNA replication. R 59022 enhances the PGF2α mitogenic response by increasing the rate of entry into the S phase. Insulin does not cause 80K phosphorylation, and does not enhance its induction but it potentiates the PGF2α mitogenic response. These results suggest that mitogenically triggered fluctuations in DAG content and PKC activity play a pivotal role in controlling the PGF2α-induced DNA synthesis while insulin acts via a different mechanism.

【 授权许可】

Unknown