Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation

FEBS Letters

de Asua, Luis Jimenez²  Goin, Mercedes¹ 

[1] Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Obligado 2490, 1428 Buenos Aires, Argentina;Department of Biochemistry, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK

关键词: Epidermal growth factor receptor;    Prostaglandin F2α;    Protein kinase C;    Diacylglycerol;    Swiss 3T3 cells;    DNA synthesis;    PGE1;    prostaglandin E1;    PGE2;    prostaglandin E2;    PGF2α;    prostaglandin F2α;    PGF2β;    prostaglandin F2β;    OAG;    I-oleoyl-2-acetylglycerol;    DAG;    diacylglycerol;    TPA;    12-O-tetradecanoyl phorbol-13-acetate;    R-59002;    (6-[2-(4-[(4-fluorophenyl) phenylmethylene]-1-piperidinyl]-7-methyl)-5H-thiazolo[3;    2-α]-pyrimidin-5-one;    R-59949;    (3-[2-[4-[bis(4-fluorophenyl)methylene]-1-piperidinyl]ethyl] 2;    3-dihydro-2-thioxo-4(1H)-quinazolinone);   

DOI  :  10.1016/0014-5793(92)80122-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostaglandin F_2α (PGF_2α) selectively decreases the binding of ¹²⁵I-labelled epidermal growth factor ([¹²⁵I]EGF) to intact Swiss 3T3 cells. Scatchard analysis reveals that PGF_2α decreases the number of high-affinity EGF binding sites without changing the total number of receptors. Prostaglandins E₁ (PGE₁), E₂ (PGE₂ or F_2β (PGF_2β) do not alter the EGF binding to these cells and do not enhance the PGF_2α effect. R-59022 and R-59949, two diacylglycerol kinase inhibitors, enhance the inhibitory effect of PGF_2α, whereas down-modulation of protein kinase C (PKC) abolishes the effect. These results indicate that PGF_2α, decreases EGF binding in Swiss 3T3 cells via PKC activation.

【 授权许可】

Unknown