Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α

FEBS Letters

de Asua, Luis Jimenez¹  Goin, Mercedes¹ 

[1] Instituto de Investigaciones en Ingenieria Genética y Biologia Molecular (INGEBI), Obligado 2490, 1428 Buenos Aires, Argentina

关键词: Swiss 3T3 cell;    Prostaglandin F2α;    Protein kinase C;    80 K phosphorylation;    DNA synthesis;    PGF2α;    prostaglandin F2α;    PGF2β;    prostaglandin F2β;    PGE1;    prostaglandin E1;    PGE2;    prostaglandin E2;    PKC;    protein kinase C;    OAG;    1-oleoyl-2-acetylglycerol;    DAG;    diacylglycerol;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    PB12;    phorbol-12;    13-dibutyrate;   

DOI  :  10.1016/0014-5793(92)80354-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostaglandin F_2α (PGF_2α), a mitogen for resting Swiss 3T3 cells, rapidly stimulates phosphorylation of an 80 kDa protein (80 K). 1-Oleoyl-2-acetylglycerol (OAG) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) both protein kinase C (PKC) activators, also elicit 80 K phosphorylation. In contrast PGE₁, PGE₂ or PGF_2β, which are non-mitogenic in these cells, had tittle or no action on this event. However PGE₁ and PGE₂ potentiate the PGF_2α proliferative effect but do not enhance its action on 80 K phosphorylation. These results suggest that PGF_2α mitogenic induction involves PKC signalling pathway activation while its enhancement by PGE₁ or PGE₂ occurs through a different mechanism(s).

【 授权许可】

Unknown