Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells

FEBS Letters

de Asua, Luis Jimenez¹  Goin, Mercedes¹  Estevez, Alvaro G.¹  Calamante, Gabriela¹ 

[1] Instituto de Investigaciones en Ingenieria Genética y Biologia Molecular (INGEBI), Obligado 2490, 1428 Buenos Aires, Argentina

关键词: Swiss 3T3 cell;    Prostaglandin F2α;    Tunicamycin;    DNA synthesis;    Lag phase;    PGF2α;    prostaglandin F2α;    TM;    tunicamycin;   

DOI  :  10.1016/0014-5793(91)81269-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tunicamycin, an inhibitor of the asparagine-linked protein N-glycosylation, blocks the initiation of DNA synthesis in Swiss 3T3 cells stimulated by prostaglandin F_2α alone or with insulin. This effect is exerted only when tunicamycin is added from 0 to 8 h after stimulation and it decreases the rate of entry into S phase. Blocking of labeled sugar incorporation to proteins occurs regardless of the time of PGF_2α stimulation. In contrast tunicamicin does not inhibit protein synthesis. These results suggest that N-glycoprotein synthesis early during the prereplicative phase is an important event controlling the mitogenic action of PGF_2α

【 授权许可】

Unknown