FEBS Letters | |
Mitogen‐stimulated events in nuclei of Swiss 3T3 cells Evidence for a direct link between changes of inositol lipids, protein kinase C requirement and the onset of DNA synthesis | |
Corps, A.N.2  Gilmour, R.S.2  Neri, L.M.3  Cocco, L.3  Manzoli, L.1  Martelli, A.M.3  | |
[1] Istituto di Morfologia Umana Normale, Universitá di Chieti, Bologna, Italy;Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK;Istitut di Anatomia Umana Normale, Unversitá di Bologna e Ferrara Italy | |
关键词: Nucleus; Mitogen; Inositol lipid; Swiss 3T3 cell; Protein kinase C; DNA synthesis; PIP; phosphatidylinositol 4-phosphate; PIP2; phosphatidylinositol 4; 5-diphosphate; PA; phosphatidic acid; BrdU; 5-bromodeoxyuridine; IGF-I; insulin-like growth factor I; IP3; inositol 1; 4; 5-trisphosphate; ATP; adenosine trisphosphate; PKC; protein kinase C; PS; phosphatidyl-serine; DAG; 1; 2-dioleyl-racglycerol); SPH; sphingosine; EGTA; ethylene glycol-bis(β-aminoethyl ether) N; N; N′; N′-tetraacetic acid; | |
DOI : 10.1016/0014-5793(91)80598-W | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two different clones of Swiss 3T3 cells belonging to the same original cell line have been obtained, one of which was unresponsive to mitogenic stimulation (e.g. insulin-like growth factor-I, bombesin, insulin-like growth factor-I + bombesin), while the other clone showed a very high rate of DNA synthesis under identical conditions as demonstrated by 5-bromodeoxyuridine incorporation. Both types of cells expressed the IGF-I receptor and showed high contact inhibition. When highly purified nuclei from responsive cells, treated for a short time with bombesin and insulin-like growth factor-I or insulin-like growth factor-I alone, were incubated with [γ-32P]adenosine triphosphate, the labelling of phosphatidylinositol-mono- und diphosphate decreased when compared to controls, while this transient effect did not appear in the nuclei from unresponsive cells. Similarly nuclear protein kinase C is activated only in responsive cells. Therefore, it seems that a direct link exists between polyphosphoinositide metabolism, protein kinase C activation and the early events leading to cell division, since the rapid changes in the labelling of both phosphatidylinositol mono- and di-phosphate occur only in nuclei from Swiss 3T3 cells, which respond to the mitogenic stimulus determined by insulin-like growth factor-I on its own, or in combination with bombesin.
【 授权许可】
Unknown
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