Abolishment of bradykinin‐induced calcium oscillations in ras‐transformed fibroblasts by the expression of 80 kDa diacylglycerol kinase

FEBS Letters

Sugimoto, Yoshikazu¹  Kanoh, Hideo²  Fu, Tao³  Okano, Yukio³  Nozawa, Yoshinori³ 

[1] Aging Process Research Laboratory (Frontier Research Program), Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Koyadai, Tsukuba, Ibaraki 305, Japan;Department of Biochemistry, Sapporo Medical College, West-17, South-1, Sapporo 060, Japan;Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan

关键词: Diacylglycerol;    Diacylglycerol kinase;    Protein kinase C;    Ca2+ oscillation;    ras-Transformed NIH/3T3 cell line (DT);   

DOI  :  10.1016/0014-5793(92)80700-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Our previous study showed bradykinin-induced periodic Ca²⁺ changes (Ca²⁺ oscillations) in v-Ki-ras-transformed NIH/3T3 (DT) cells in which protein kinase C (PKC) activity is partially down-regulated by a sustained high level of 1,2-diacylglycerol (DAG) [FEBS Lett. (1991) 281. 263–266].In the present study, DAG kinase with 80 kDa mass (80K DGK) has been successfully transfected in DT cells, which exhibited enhanced cellular DAG kinase activities, decreased cellular DAG contents, and increased PKC activities compared to the control vector-transfected cells. Furthermore, these DGK-transfectants showed strong inhibition in bradykinin-induced Ca²⁺ oscillations. The results suggest that the sustained DAG increase down-regulates the PKC activity, thereby leading to the induction of Ca²⁺ oscillations in DT cells.

【 授权许可】

Unknown