Effect of perturbing diacylglycerol metabolism on cytosolic free Ca2+ oscillations induced in single hepatocytes

FEBS Letters

Sanchez-Bueno, Antonio¹  Cobbold, Peter H.¹ 

[1] Department of Human Anatomy and Cell Biology, The University of Liverpool, Liverpool L69 3BX, UK

关键词: Ca2+ oscillation;    Diacylglycerol;    R 59 022;    diC8;    Single hepatocyte;    free Ca;    cytosolic concentration of free Ca2+;    DG;    sn-1;    2-diacylglycerol;    diC8;    sn-1;    2-dioctanoylglycerol;   

DOI  :  10.1016/0014-5793(92)81409-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca²⁺-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC₈) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These results agree with a negative feedback effect of protein kinase C on free Ca oscillations.

【 授权许可】

Unknown