Stereospecific bioactions of 5‐hydroxyicosatetraenoate

FEBS Letters

Rossi, Adriano G.¹  Thomas, Michael J.²  O'Flaherty, Joseph T.¹ 

[1] Department of Medicine, Wake Forest University Medical Center, Winston-Salem, NC 27103, USA;Department of Biochemistry, Wake Forest University Medical Center, Winston-Salem, NC 27103, USA

关键词: 5-Hydroxyicosatetraenoate;    cellular Ca2+;    Degranulation;    Polymorphonuclear neutrophil;    5(S)-HETE;    5(S)-hydroxy-6;    8;    11;    14-E;    Z;    Z;    Z-eicosatetraenoate;    5(R)-HETE;    (5(R)-hydroxy-6;    8;    11;    14-E;    Z;    Z;    Z-eicosatetraenoate;    LTB4;    leukotriene B4;    PAF;    platelet-activating factor;    PMA;    4β-phorbol-12-myristate-13-acetate;    diC8;    1;    2-dioctanoylglycerol;    BSA;    bovine serum albumin;    [Ca2+]i;    cytosolic free Ca2+ concentration;   

DOI  :  10.1016/0014-5793(88)80360-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20–100-fold weaker than the natural isomer in these assays. Thus, the arachidonic acid metabolite activates PMNs by a stereospecific possibly receptor-mediated mechanism.

【 授权许可】

Unknown