Selenium added unripe carica papaya pulp extracts enhance wound repair through TGF-β1 and VEGF-a signalling pathway

BMC Complementary and Alternative Medicine

Research Article

Mohammad Tariqur Rahman¹  Abdulrazaq Bidemi Nafiu² 

[1] Faculty of Dentistry, University Malaya, 50603, Kuala Lumpur, Malaysia;Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Pahang, Kuantan, Malaysia;

关键词: Collagen synthesis;    Transforming growth factor (TGF);    Vascular endothelial growth factor (VEGF);    Polymorphonuclear neutrophil;    Fibroblasts;    Wound repair;    Inflammation;   

DOI  :  10.1186/s12906-015-0900-4

 received in 2015-07-11, accepted in 2015-10-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIncreased wound healing efficiency by Se2+ added Carica papaya L. (Caricaceae) fruit extract was linked to increased antioxidant and anti-inflammatory responses during healing.We investigated the impact of Se2+ or Zn2+ added papaya water (WE) and phosphate-buffered saline (PE) extracts on cells recruitment and bio-molecular alterations on days 4 and 10 post wounding in an in vivo excision wound.MethodsExcision wounds were created on the dorsum of Sprague Dawley rats and treated topically twice/day with 20 μL of PE and WE (5 mg extract/mL), 0.5 μgSe2+ added PE and WE (PES and WES), or 100 μMZn2+ added PE and WE (PEZ and WEZ). Deionised water (negative) and Solcoseryl (positive) were applied on the control groups. Histochemical and biochemical assays were used to evaluate cellular and bio-molecular changes in the wound.ResultsPES (PE + 0.5 μg Se2+) only increased significantly (p < 0.05) wound total protein content (95.14 ± 1.15 mg/g tissue vs positive control; 80.42 ± 0.86 mg/g tissue) on day 10 post wounding. PES increased significantly (p < 0.05) the number of fibroblasts/high power field (HPF) (75.60 ± 9.66) but decreased significantly (p < 0.05) the number of polymorphonuclear leukocytes/HPF (59.20 ± 12.64) in the wound compared to positive control (50.60 ± 12.58 fibroblasts/HPF, 101.00 ± 27.99 polymorphonuclear leukocytes/HPF) on day 4. Similar results were recorded for WES. PES demonstrated increased neovascularization, TGF-β1 and VEGFA expressions at day 4 and increased collagen at day 10.ConclusionPapaya extract improved wound repair by increasing fibroblasts recruitment and reducing polymorphonuclear leukocytes infiltration through early transient expressions of TGF-β1 and VEGFA at the wound area. The processes were amplified with Se2+ addition.

【 授权许可】

CC BY   
© Nafiu and Rahman. 2015