Immunogold localisation of ubiquitin‐protein conjugates in primary (azurophilic) granules of polymorphonuclear neutrophils

FEBS Letters

Doherty, Fergus J.³  Mayer, R.John³  László, Lajos¹  Landon, Michael³  Self, Tim²  Lowe, James⁴  Watson, Adrian³ 

[1] Department of General Zoology, Eötvös University, Puskin u.3, H-1088 Budapest, Hungary;Department of Human Morphology, University of Nottingham Medical School, Queens Medical Centre, Nottingham, NG7 2UH, UK;Department of Biochemistry, University of Nottingham Medical School, Queens Medical Centre, Nottingham, NG7 2UH, UK;Department of Pathology, University of Nottingham Medical School, Queens Medical Centre, Nottingham, NG7 2UH, UK

关键词: Ubiquitin;    Azurophilic granule;    Lysosome;    Polymorphonuclear neutrophil;   

DOI  :  10.1016/0014-5793(91)80142-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ubiquitin—protein conjugates are found in the primary (azurophilic) lysome-related granules but not in the secondary (specific) granules in mature polymorphonuclear neutrophils prepared from bone marrow. This is the first reported demonstration of ubiquitin—protein conjugates in lysosome-related membrane-bound vesicles in granulocytes and complements our previous findings of ubiquitinated proteins in lysosomes of fibroblasts. The significance of the selective presence of conjugates in only one of the two main types of neutrophil granules remains to be elucidated but may relate to the presence of the complement of acid hydrolases, including proteases, in the azurophilic granules compared to the specific granules. Ubiquitin—protein conjugates may enter the primary granules during neutrophil maturation by an autophagic process or by a heterophagic process during the fusion of phagosomes with primary granules. Alternatively protein ubiquitination may be involved in granule biogenesis.

【 授权许可】

Unknown