Calcium oscillation associated with reduced protein kinase C activities in ras‐transformed NIH3T3 cells

FEBS Letters

Sugimoto, Yoshikazu²  Fu, Tao¹  Murakami, Shigeko²  Okano, Yukio¹  Nozawa, Yoshinori¹  Oki, Tohru² 

[1] Department of Biochemistry, Gifu University School of Medicine, Gifu 500, Japan;Aging Process Research Laboratory (Frontier Research Program), Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Tsukuba, Ibaraki 305, Japan

关键词: Ca2+ oscillation;    ras-Transformed fibroblast;    Protein kinase C;    BK;    bradykinin;    [Ca2+]i;    intracellular Ca2+ concentration;    EGTA;    [ethylenebis(oxyethylene nitrilo)]tetraacetic acid;    fura-2/AM;    fura-2 acetoxymetyl ester;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid;    H-7;    1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride;    IP3(1;    4;    5);    inositol 1;    4;    5-trisphosphate;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;   

DOI  :  10.1016/0014-5793(91)80407-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show here novel intracellular Ca²⁺ oscillation in ν-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca²⁺ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca²⁺ oscillation is negatively regulated by PKC, which modulates Ca²⁺ influx in ν-K-ras-transformed NIH3T3 cells.

【 授权许可】

Unknown