Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca2+‐induced Ca2+ release and Ca2+ oscillations in hamster eggs

FEBS Letters

Yuzaki, Michisuke¹  Miyazaki, Shun-ichi⁴  Shirakawa, Hideki⁴  Nakada, Ken⁴  Mikoshiba, Katsuhiko²  Honda, Yoshiko⁴  Nakade, Shinji³ 

[1] Department of Biochemistry, Jichi Medical School, Tochigi-ken, Japan;Department of Molecular Neurobiology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan;Division of Regulation of Macromolecular Function, Institute for Protein Research, Osaka University, Sulta, Japan;Department of Physiology, Tokyo Women's Medical College, Kawada-cho, Shinjuku-ku, Tokyo, Japan

关键词: Sulfhydryl reagent;    Anti-InsP3 receptor antibody;    Ca2+-induced Ca2+ release;    InsP3-induced Ca2+ release;    Ca2+ oscillation;    Hamster egg;   

DOI  :  10.1016/0014-5793(92)81090-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sulfhydryl reagent thimerosal enhanced the sensitivity of hamster eggs to injected inositol 1,4,5-trisphosphate (InsP₃) or Ca²⁺ to generate regenerative Ca²⁺ release from intracellular pools. A monoclonal antibody (mAb) to the InsP₃ receptor blocked both the InsP₃-induced Ca²⁺ release (IICR) and Ca²⁺-induced Ca²⁺ release (CICR). The mAb also blocked Ca²⁺ oscillations induced by thimerosal. The results indicate that thimerosal enhances IICR sensitized by cytosolic Ca²⁺, but not CICR from InsP₃-insensitive pools, and causes repetitive Ca²⁺ releases from InsP₃-sensitive pools.

【 授权许可】

Unknown