Ca2+‐induced Ca2+ release in insulin‐secreting cells

FEBS Letters

Rorsman, Patrik¹  Berggren, Per-Olof¹  Islam, Md.Shahidul¹ 

[1] The Rolf Luft Center for Diabetes Research, Department of Endocrinology, Karolinska Institute, Box 60500, Karolinska Hospital, S-104 01 Stockholm, Sweden

关键词: Ca2+-induced Ca2+ release;    Sulphydryl reagent;    Thimerosal;    Intracellular Ca2+ transport;    Insulin-secreting cell;    Permeabilized cell;   

DOI  :  10.1016/0014-5793(92)80306-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sulphydryl reagent thimerosal (50 μM) released Ca²⁺ from a non-mitochondrial intracellular Ca²⁺ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca²⁺ was released from an Ins(1,4,5)P₃-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P₃-sensitive pool by a supramaximal dose of Ins(2,4,5)P₃ or thapsigargin. The Ins(1,4,5)P₃-sensitive pool remained essentially unaltered by thimerosal. Thimerosal-induced Ca²⁺ release was potentiated by caffeine. These findings suggest the existence of Ca²⁺-induced Ca²⁺ release also in insulin-secreting cells.

【 授权许可】

Unknown