The modulation and characterisation of the Ca2+‐induced Ca2+ release mechanism in cultured human myometrial smooth muscle cells

FEBS Letters

Morgan, J.M.¹  Gillespie, J.I.¹ 

[1] Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK

关键词: Smooth muscle;    Ca2+-induced Ca2+ release;    Human myometrium;   

DOI  :  10.1016/0014-5793(95)00771-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The process of Ca²⁺-induced Ca²⁺ release (CICR) was studied in saponin permeabilised human myometrial smooth muscle cells in which the sarcoplasmic reticulum (SR) was pre-loaded with ⁴⁵Ca²⁺. A rise in the free Ca²⁺ concentration of the bathing solution from 100 nM tot 10 μM increased the rate of ⁴⁵Ca²⁺ loss, while a reduction to 10 nM decreased the rate of ⁴⁵Ca²⁺ loss. Ruthenium red (20 μM) lowered the basal rate of ⁴⁵Ca²⁺ loss and reduced CICR. Caffeine did not activate ⁴⁵Ca²⁺ release although ryanodine induced ⁴⁵Ca²⁺ release and CICR was augmented in the presence of caffeine. These data suggest the operation of a Ca²⁺-activated ⁴⁵Ca²⁺ release process which is similar in many of its properties to the CICR process described in many cells. The basic properties of the CICR mechanism in myometrium differs in many respects from the CICR process recently described in human vascular smooth muscle.

【 授权许可】

Unknown