Expression of a cDNA encoding the glycolipid‐anchored form of rat acetylcholinesterase

FEBS Letters

Massoulié, Jean¹  Legay, Claire¹  Bon, Suzanne¹ 

[1] Laboratoire de Neurobiologie, CNRS UA 295, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cédex 05, France

关键词: Acetylcholinesterase;    Glycolipid anchor;    Rat;    Transfection;    AChE;    acetylcholinesterase;    GPI;    glycophosphatidylinositol.;   

DOI  :  10.1016/0014-5793(93)81155-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We amplified by PCR and characterized a fragment of cDNA from rat spleen, encoding the distinctive C-terminal region of the acetylcholinesterase (AChE) H subunit. A recombinant vector encoding this subunit was constructed and expressed in COS cells: the H subunits produced glycophosphatidylinositol (GPI)-anchored dimers, showing that the spleen cDNA fragment contained a functional GPI cleavage/attachment site. Using PCR, we did not detect mRNAs encoding AChE H in rat muscle or hypothalamus. In the liver of 16-day rat embryos, we found both H and T transcripts, in agreement with the presence of both GPI-anchored dimers and amphiphilic monomers of type II. In addition, we detected ‘read-through’ (R) transcripts, in which regular introns are spliced, but the intervening sequence between the common exon 4 and the alternative exon 5 (H) is maintained.

【 授权许可】

Unknown