Internucleosomal chromatin degradation in myeloma and B‐hybridoma cell cultures

FEBS Letters

Khodarev, Nikolai N.²  Volgina, Veronica V.¹  Sokolova, Irina A.¹  Makarova, Natalia V.¹  Volgin, Andrei U.¹  Shishkin, Sergei S.¹ 

[1] National Research Center of Medical Genetics, Russian Academy of Medical Sciences, Moscow, Russian Federation;Center of Medical Biotechnology, Russian Ministry of Health, Moscow, Russian Federation

关键词: Hybridoma;    Apoptosis;    Ca/Mg-dependent endonuclease;   

DOI  :  10.1016/0014-5793(92)81213-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c is compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isolated cell nuclei of X-63 the activation of CME, which precedes chromatin fragmentation in vivo and loss of cell viability, is revealed. The time—course of CME activation is opposite to cell proliferation and is not accompanied by alterations in enzyme quantity. The results suggest that cell death of X-63 and MLC-1c occurs via apoptosis, and involves the mechanisms controlling the activation and/or interaction of CME with chromatin.

【 授权许可】

Unknown