Presence of a novel subset of NKT cells bearing an invariant Vα19.1–Jα26 TCR α chain

FEBS Letters

Shimamura, Michio¹  Huang, Yi-Ying¹ 

[1] Laboratory of Developmental Immunology, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan

关键词: Natural killer T cell;    Invariant TCR α chain;    V–J junction;    Hybridoma;    Interleukin-4 secretion;    NKT;    natural killer T;    β2m;    β2-microglobulin;    DN;    CD4−CD8− double negative;    Tap;    transporter associated with antigen processing;    RACE;    rapid amplification of cDNA ends;    DIG;    digoxigenin;    HPRT;    hypoxanthine phosphoribosyltransferase;   

DOI  :  10.1016/S0014-5793(02)02509-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD1d-deficient (CD1d^−/−) mouse lymphocytes were analyzed to classify the natural killer T (NKT) cells without reactivity to CD1d. The cells bearing a Vα19.1–Jα26 (AV19–AJ33) invariant TCR α chain, originally found in the peripheral blood lymphocytes, were demonstrated to be abundant in the NK1.1⁺ but not NK1.1⁻ T cell population isolated from CD1d^−/− mice. Moreover, more than half (11/21) of the hybrid cell lines established from CD1d^−/− NKT cells expressed the Vα19.1–Jα26 invariant TCR α chain. The expression of the invariant Vα19.1–Jα26 mRNA was absent in β2-microglobulin-deficient mice. Collectively, the present findings suggest the presence of a second NKT cell repertoire characterized by an invariant TCR α chain (Vα19.1–Jα26) that is selected by an MHC class I-like molecule other than CD1d.

【 授权许可】

Unknown