Cell cycle kinetics, tissue transglutaminase and programmed cell death (apoptosis)

FEBS Letters

El Alaoui, S.¹  Quash, G.²  Mian, S.¹  Griffin, M.¹  Lawry, J.³ 

[1] Dept. of Life Sciences, Nottingham Polytechnic, Nottingham, Clifton Lane, NG11 8NS, UK;Laboratoire d'Immunochimie INERM C.J.F. 89-05, Faculté de Medecine Lyon-Sud, Chemin du Petit Revoyet, 69921 Oullins, France;Dept. of Experimental and Clinical Microbiology, University Medical School, Beech Hill Road, Sheffield S10 2RX, UK

关键词: Cell cycle;    Tissue transglutaminase;    Apoptosis;   

DOI  :  10.1016/0014-5793(92)81392-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Studies were undertaken on a highly metastatic hamster fibrosarcoma cell line with a view to assessing whether cells entering into apoptosis, measured by counting the number of transglutaminase mediated detergent insoluble envelopes, has any synchrony with a particular phase of the cell cycle. A double exposure of thymidine was used to block cells in early S-phase. Flow cytometry in combination with [³H]thymidine incorporation into DNA was used to assess the degree of synchrony and progression through the different phases of cell cycle. The apoptotic index was found to be at its maximum in mid-S-phase, Measurement of transglutaminase activity in each phase of the cell cycle indicated that the specific activity was also at its greatest during mid S-phase. The level of enzyme was relatively unchanged throughout the cell cycle indicating that the regulation of transglutaminase activity occurs primarily through effects on catalytic activity rather than enzyme synthesis.

【 授权许可】

Unknown