Multiple cell cycle access to the apoptotic death programme in human neuroblastoma cells

FEBS Letters

Piacentini, Mauro¹  Fesus, Laszlo²  Melino, Gerry³ 

[1] Department of Biology, University of Rome ‘Tor Vergata’ Rome, Italy;Department of Biochemistry, University Medical School of Debrecen, Debrecen, Hungary;Department of Experimental Medicine and Biochemical Sciences, University of Rome ‘Tor Vergata’ Rome, Italy

关键词: Apoptosis;    Neuroblastoma;    Cisplatin;    Tissue transglutaminase;    tTG;    ‘tissue’ transglutaminase;    RA;    retinoic acid;    MEM;    minimal essential medium;    HEPES;    N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid;    PBS;    phosphate-buffered saline;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(93)80081-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the induction of apoptosis in a human neuroblastoma cell line SK-N-BE(2) by cisplatin or retinoic acid, and its relation to cell cycle. Apoptosis was monitored by counting apoptotic bodies and evaluating the activity of ‘tissue’ transglutaminase (EC 2.3.2.13), one of the genes specifically expressed in apoptotic cells. Data indicate that both agents enhance apoptosis, even though cells arrest at different cell cycle phases. In fact, retinoic acid causes accumulation in G₁, whilst cisplatin induces accumulation of cells in the G₂/M phase. This evidence suggests the presence of multiple start points for the apoptotic death programme within the cell cycle of human neuroblastoma cells.

【 授权许可】

Unknown