Reduced puromycin sensitivity of translocated polysomes after the addition of elongation factor 2 and non‐hydrolysable GTP analogues

FEBS Letters

Nilsson, Lars¹  Nygård, Odd¹ 

[1] Department of Cell Biology, Arrhenius Laboratories E5, Stockholm University, Stockholm, Sweden

关键词: Elongation factor;    Eukaryote;    Protein synthesis;    Puromycin;    Reticulocyte;    Translocation;    GuoPP[CH2]P;    guanosine 5′-[β;    -τ-methylene]-triphosphate;    cEF;    eukaryotic elongation factor;    EF;    prokaryotic elongation factor;   

DOI  :  10.1016/0014-5793(92)80746-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of reticulocyte polysomes with elongation factor cEF-2 and GTP led to an increased sensitivity or peptidyl-tRNA for puromycin as a result of the translocation from the ribosomal A-site to the P-site, upon addition of an excess of the non-hydrolysable GTP analogue, GuoPP(CH₂]P, the puromycin sensitivity decreased rapidly, The decrease in sensitivity required high concentrations of cEF-2 with half maximal effect at an cEF-2 concentration of around 1 μM. The data suggest either that peptidyl-tRNA had re-translocated back to the A-site due to the higher affinity of cEF-2 for the pre-translocation than for the post-translocation ribosome, or that the cEF-2-GuoPP[CH₂]P complex blocks the peptidyl-transferase activity.

【 授权许可】

Unknown