FEBS Letters | |
Reduced puromycin sensitivity of translocated polysomes after the addition of elongation factor 2 and non‐hydrolysable GTP analogues | |
Nilsson, Lars1  Nygård, Odd1  | |
[1] Department of Cell Biology, Arrhenius Laboratories E5, Stockholm University, Stockholm, Sweden | |
关键词: Elongation factor; Eukaryote; Protein synthesis; Puromycin; Reticulocyte; Translocation; GuoPP[CH2]P; guanosine 5′-[β; -τ-methylene]-triphosphate; cEF; eukaryotic elongation factor; EF; prokaryotic elongation factor; | |
DOI : 10.1016/0014-5793(92)80746-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Treatment of reticulocyte polysomes with elongation factor cEF-2 and GTP led to an increased sensitivity or peptidyl-tRNA for puromycin as a result of the translocation from the ribosomal A-site to the P-site, upon addition of an excess of the non-hydrolysable GTP analogue, GuoPP(CH2]P, the puromycin sensitivity decreased rapidly, The decrease in sensitivity required high concentrations of cEF-2 with half maximal effect at an cEF-2 concentration of around 1 μM. The data suggest either that peptidyl-tRNA had re-translocated back to the A-site due to the higher affinity of cEF-2 for the pre-translocation than for the post-translocation ribosome, or that the cEF-2-GuoPP[CH2]P complex blocks the peptidyl-transferase activity.
【 授权许可】
Unknown
【 预 览 】
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