Species‐specific differences in the toxicity of puromycin towards cultured human and Chinese hamster cells

FEBS Letters

Dudani, Anil K.¹  Gupta, Radhey S.¹  Gupta, Rajni¹ 

[1] McMaster University, Department of Biochemistry, 1200 Main Street W., Hamilton, Ontario L8N 3Z5, Canada

关键词: Cellular toxicity;    Puromycin;    Species difference;    (Mammalian cell);   

DOI  :  10.1016/0014-5793(88)81320-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The toxicity of the protein synthesis inhibitor puromycin towards a number of human and Chinese hamster cell lines has been examined. In comparison to cells of human origin, Chinese hamster cells exhibited about 25-fold higher resistance towards puromycin. These differences appeared to be species related as all the cell lines from any one species showed similar sensitivity towards puromycin. The incorporation of [³H]leucine in the hamster cell lines was accordingly found to be more resistant to the inhibitory effects of puromycin as compared to human cells. Studies on the cellular uptake of [³H]puromycin showed that in comparison to human cells, the drug uptake/binding in the hamster cell lines was greatly reduced. However, protein synthesis in the extracts of hamster and human cells showed no significant differences in sensitivity towards puromycin. These results show that the observed species related differences in cellular toxicity to puromycin are due to differences in the cellular uptake/binding of the drug.

【 授权许可】

Unknown