Protein synthesis in HL‐60 cells treated with DMSO and hypoxanthine

FEBS Letters

Masemola, A.M.¹  Tyobeka, E.M.¹ 

[1] Department of Biochemistry, University of the North, Private Bag XI106, Sovenga 0727, South Africa

关键词: Protein synthesis;    tRNA;    DMSO;    Hypoxanthine;    FBS;    fetal bovine serum;    DMSO;    dimethylsulfoxide;    eIF-2;    eukaryotic initiation factor 2;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(92)81267-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Short-term treatment of the HL-60 cells with DMSO and hypoxanthine, inducers of granulocytic differentiation, was reported to cause a rapid increase in protein synthesis. This effect was ascribed to the insertion of inosine in the wobble position of the tRNA anticodon and consequently increasing codon recognition potential. In this study we have re-investigated the effects of DMSO and/or hypoxanthine on protein synthesis. In contrast to their findings we were unable to demonstrate stimulated protein synthesis in either short- or long-term treatment with these agents. Polysome analysis under these conditions revealed that polysomes were disaggregated. Finally, the activity of tRNA-hypoxanthine ribosyltransferase, an enzyme responsible for the insertion of inosine in the anticodon, was also relatively low. Under these circumstances, we propose that tRNA modification is not essential in the regulation of protein synthesis.

【 授权许可】

Unknown