Protein synthesis in rat cardiac myocytes is stimulated at the level of translation by phorbol esters

FEBS Letters

Sugden, Peter H.¹  Fuller, Stephen J.¹ 

[1] Department of Cardiac Medicine, National Heart and Lung Institute, University of London, Dovehouse Street, London SW3 6LY, England

关键词: Protein synthesis;    Phorbol ester;    Translational control;    (Rat heart myocyte);    AM-D;    actinomycin D;    BSA;    bovine serum albumin;    DMSO;    dimethyl sulphoxide;    K-H;    Krebs and Henseleit;    PDB;    phorbol 12;    13-dibutyrate;    PDD;    phorbol 12;    13-didecanoate;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(89)81336-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

12-O-Tetradecanoylphorbol 13-acetate acutely stimulated the rate of protein synthesis maximally by about 43% in terminally differentiated myocytes freshly isolated from adult rat hearts. Stimulation was rapidly expressed (within 20 min). The relative effects of four phorbol esters on protein synthesis was consistent with a specific effect on protein kinase C. Inhibition of transcription with actinomycin D had no effect on the absolute stimulation of the protein synthesis rate by 12-O-tetradecanoylphorbol 13-acetate. We conclude that protein kinase C may be involved in the regulation of the translational process.

【 授权许可】

Unknown