Equilibration of methionine into the Met‐tRNA pool of rabbit reticulocyte lysates

FEBS Letters

Gross, Martin¹  Knish, Walter M.¹  Kaplansky, Daniel A.¹ 

[1] Department of Pathology, The University of Chicago, 950 East 59th Street, Chicago, IL 60637, USA

关键词: Reticulocyte lysate;    Hemin;    Protein synthesis;    Translational control;    Deacylation;    Met-tRNA;    HCR;    hemin-controlled translational repressor;    dsI;    double-stranded RNA-activated inhibitor;   

DOI  :  10.1016/0014-5793(83)80976-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have measured the rate of equilibration of [³⁵S]methionine into the Met-tRNA pool of rabbit reticulocyte lysate as in [FEBS Lett. (1982) 143, 301–305]. Our results indicate that hemin-deficiency inhibits the equilibration of methionine into the tRNA pool much less than protein synthesis or the equilibration of alanine into the tRNA pool, whereas cycloheximide inhibits these processes similarly. This finding is consistent with our previous data and supports the hypothesis that with hemin-deficiency much of the Met-tRNA_f that becomes bound to 40 S subunits subsequently undergoes enzymatic deacylation.

【 授权许可】

Unknown