CpA containing oligoribonucleotides specifically inhibit protein synthesis in rabbit reticulocytes

FEBS Letters

Wagner, Thomas¹  Gross, Martin²  Sigler, Paul B.¹ 

[1] Departments of Biochemistry & Molecular Biology, The University of Chicago, 920 E. 58th Street, Chicago, IL 60637, USA;Departments of Pathology, The University of Chicago, 920 E. 58th Street, Chicago, IL 60637, USA

关键词: Oligoribonucleotide;    Reticulocyte lysate;    Protein synthesis;    3'-Exonucleolytic cleavage;    Cell-free system;    (Rabbit;    Wheat germ);   

DOI  :  10.1016/0014-5793(87)81368-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The diribonucleoside monophosphate CpA (and no others) inhibits polypeptide chain elongation in rabbit reticulocyte lysates at 10–50 μM. Furthermore, all the trinucleotides containing CpA, i.e., XpCpA and CpApX (X = U, C, A or G) block polypeptide chain elongation as well. At 10 μM the inhibition by XpCpA and not CpApX is transient because a 3'-exonucleolytic activity destroys the critical CpA moiety. The inhibitors do not appear to interfere with the aminoacylation of tRNAs or disrupt the interaction of aminoacyl-tRNAs with the protein synthetic machinery. High levels (200 μM) of CpA or the trinucleotides containing CpA have no effect on translation in a wheat germ cell-free system.

【 授权许可】

Unknown