A new endogenous differentiating factor (myelopeptide‐4) for myeloid cells

FEBS Letters

Fonina, Larissa A¹  Mikhailova, Augusta A¹  Petrov, Rem V¹  Strelkov, Leonid A¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 16/10 Miklukho-Maklaya, 117871 Moscow, Russia

关键词: Myelopeptide;    HL-60 cell;    K-562 cell;    DNA synthesis;    Protein synthesis;    CD14 antigen;    Differentiation;    CM;    conditioned medium;    DMSO;    dimethylsulfoxide;    MI;    maturation inducer;    MP;    myelopeptide;    PMA;    phorbol myristate acetate;   

DOI  :  10.1016/S0014-5793(00)01344-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Along with known lymphokines involved in the regulation of hematopoiesis, a new differentiating factor (myelopeptide-4, MP-4) for myeloid cells was found. The peptide (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro) originally isolated from the culture medium of porcine bone marrow cell culture was examined for its ability to induce differentiation in two human myeloid leukemia cell lines, HL-60 and K-562. Agents with well-known differentiation-inducing activity, such as phorbol myristate acetate, dimethylsulfoxide and the lymphokines were used as a reference. It has been shown that MP-4 significantly influences the integral characteristics of metabolism, expression of surface antigens and morphology of these cells. It decreased the level of chromosomal DNA synthesis and, in parallel, increased the total protein synthesis in both HL-60 and K-562 cells. MP-4 induced the expression of CD14 monocyte-specific surface antigen and the appearance of mature monocytes/macrophages in HL-60 cell cultures. There was a good correlation of cell metabolic/morphological changes and the CD14 marker expression for HL-60 cells. A similar phenomenon was observed in K-562 cells treated with MP-4 when the levels of hemoglobin synthesis were detected in their cytoplasm. Thus, we consider MP-4 as a new endogenous differentiating factor for myeloid cells.

【 授权许可】

Unknown