The activation of nuclear phosphoinositide 3‐kinase C2β in all‐trans‐retinoic acid‐differentiated HL‐60 cells

FEBS Letters

Banfić, Hrvoje¹  Batinić, Drago³  Crljen, Vladiana¹  Višnjić, Dora¹  Ćurić, Josip³  Volinia, Stefano² 

[1] Department of Physiology and Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Šalata 3, 10 000 Zagreb, Croatia;Dipartimento di Morfologia ed Embriologia, Universita degli Studi, Via Fossato di Mortara 64/b, 44100 Ferrara, Italy;Department of Clinical Laboratory Diagnosis, Clinical Hospital Center, 10 000 Zagreb, Croatia

关键词: Phosphoinositide 3-kinase C2β;    Nucleus;    HL-60 cell;    All-trans-retinoic acid;    Tyrosine phosphorylation;    PI3K;    phosphoinositide 3-kinase;    PtdIns;    phosphatidylinositol;    PtdIns(3)P;    phosphatidylinositol 3-phosphate;    PtdIns(4)P;    phosphatidylinositol 4-phosphate;    PtdIns(3;    4)P2;    phosphatidylinositol 3;    4-bisphosphate;    PtdIns(4;    5)P2;    phosphatidylinositol 4;    5-bisphosphate;    PtdIns(3;    4;    5)P3;    phosphatidylinositol 3;    4;    5-trisphosphate;    PtdSer;    phosphatidylserine;    ATRA;    all-trans-retinoic acid;    IFN-γ;    interferon-γ;    DMSO;    dimethyl sulfoxide;    1;    25(OH)2D3;    1;    25-dihydroxyvitamin D3;    db cAMP;    dibutyryl-adenosine 3′;    5′-cyclic monophosphate;    PMA;    phorbol 12-myristate 13-acetate;    EGFR;    epidermal growth factor receptor;    PDGFR;    platelet-derived growth factor receptor;   

DOI  :  10.1016/S0014-5793(02)03357-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activity of nuclear phosphoinositide 3-kinase C2β (PI3K-C2β) was investigated in HL-60 cells induced to differentiate along granulocytic or monocytic lineages. A significant increase in the activity of immunoprecipitated PI3K-C2β was observed in the nuclei and nuclear envelopes isolated from all-trans-retinoic acid (ATRA)-differentiated cells which was inhibited by the presence of PI3K inhibitor LY 294002. High-performance liquid chromatography analysis of inositol lipids showed an increased incorporation of radiolabelled phosphate in both PtdIns(3)P and PtdIns(3,4,5)P₃ with no changes in the levels of PtdIns(4)P, PtdIns(3,4)P₂ and PtdIns(4,5)P₂. Western blot analysis of the PI3K-C2β immunoprecipitates with anti-P-Tyr antibody revealed a significant increase in the level of the immunoreactive band corresponding to PI3K-C2β in the nuclei and nuclear envelopes isolated from ATRA-differentiated cells.

【 授权许可】

Unknown