Induction of thromboxane synthase and prostaglandin endoperoxide synthase mRNAs in human erythroleukemia cells by phorbol ester

FEBS Letters

Tanabe, Tadashi²  Miyata, Atsuro²  Nüsing, Rolf¹  Ullrich, Volker¹  Yokoyama, Chieko²  Ihara, Hayato²  Kosaka, Tetsuya² 

[1] Faculty of Biology, PO Box 5560, University of Konstanz, D-7750 Konstanz, Germany;Department of Pharmacology, National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

关键词: Thromboxane synthase;    Prostaglandin endoperoxide synthase;    12-O-Tetradecanoyl-phorbol-13-acetate;    TXS;    thromboxane syrthase;    PES;    prostaglandin endoperoxide synthase;    HEL cells;    human crythroleukemia cells;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;   

DOI  :  10.1016/0014-5793(92)80990-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the mRNA levels of two enzymes, thromboxane synthase (TXS) and prostaglandin endoperoxide synthase (PES), responsible for the synthesis of thromboxane A₂ from arachidonic acid, were studied in human crythroleukemia (HEL) cells by RNA blot analysis. TPA induced both TXS and PES mRNAs in HEL cells in a dose-dependent manner at 36 h. The half-maximal and maximal effects for the induction of both mRNAs ware at approximately 3×10⁻⁹ M and at 10⁻⁸ M, respectively. TXS and PES mRNA levels increased in a time-dependent fashion by TPA, and reached to 7- and 3.5-fold of the control, respectively after 48 h of TPA treatment. These results suggest that expression of TXS and PES genes in HEL cells were simultaneously stimulated by TPA.

【 授权许可】

Unknown