Primary structure of sheep prostaglandin endoperoxide synthase deduced from cDNA sequence

FEBS Letters

Tanabe, Tadashi¹  Yokoyama, Chieko¹  Takai, Toshiyuki¹ 

[1] Laboratory of Cell Biology, National Cardiovascular Center Research Institute, Fujishiro-dai, Suita, Osaka 565, Japan

关键词: Prostaglandin endoperoxide synthase;    Cyclooxygenase;    cDNA cloning;    Nucleotide sequence;    Primary structure;    (Sheep vesicular gland);   

DOI  :  10.1016/0014-5793(88)80847-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact enzyme polypeptide and peptide fragments obtained by limited proteolysis of the enzyme with Achromobacter proteinase I. Mature sheep prostaglandin endoperoxide synthase is shown to be composed of 576 amino acids with an M _r of 66 175. The precursor peptide is predicted to contain a 24-residue signal peptide. The serine residue susceptible to acetylation by aspirin is found to be located near the C-terminus of the enzyme polypeptide.

【 授权许可】

Unknown