Molecular cloning and sequence analysis of cDNA encoding human kidney D‐amino acid oxidase

FEBS Letters

Miyake, Yoshihiro¹  Momoi, Kyoko¹  Watanabe, Fusao¹  Fukui, Kiyoshi¹ 

[1] Department of Biochemistry, National Cardiovascular Center Research Institute, Fujishirodai 5-chome, Suita, Osaka 565, Japan

关键词: D-Amino acid oxidase;    cDNA cloning;    Nucleotide sequence;    (Human);   

DOI  :  10.1016/0014-5793(88)80252-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones encoding D-amino acid oxidase were isolated from a human kidney cDNA library by hybridization with cDNA for the pig enzyme. The cDNA insert of 2.0 kilobase pairs long provided coding information for a protein consisting of 347 amino acids. The molecular mass of the enzyme was calculated to be 39 410 Da. The amino acid sequence similarity between the pig and human enzymes is 84.4%, and among the active site residues proposed from chemical modification studies, methionine-110 of the pig enzyme was replaced by threonine. Northern blot analysis confirmed the expression of an mRNA of 2.0 kilobases encoding the D-amino acid oxidase in human kidney.

【 授权许可】

Unknown